期刊论文

Effects of Buyang Huanwu decoction on expressions of nuclear factor-κBp65 and its inhibitor in rats with focal cerebral ischemia injury

中文

中国中西医结合急救杂志

1008-9691

2014

3

161-164

10.3969/j.issn.1008-9691.2014.03.001

81072939:国家自然科学基金 81302953:国家自然科学基金 13BO82:教育厅项目 09K056:湖南省高等学校创新平台开放基金 10K048:湖南省高等学校创新平台开放基金

本校为第一机构

目的：探讨补阳还五汤对脑缺血大鼠脑组织核转录因子-κB（NF-κB）信号通路NF-κBp65及其抑制物（I-κB）表达的影响。方法将180只SD大鼠按随机数字表法分为正常对照组、假手术组、模型组、吡咯烷二硫代氨基甲酸盐（PDTC）组、米诺环素和补阳还五汤组，每组30只。PDTC组腹腔注射100 mg?kg-1?d-1 PDTC；米诺环素组给予米诺环素2.35 g?kg-1?d-1加蒸馏水按相同体积配成各浓度药液灌胃；补阳还五汤组给予补阳还五汤，按人与动物体表面积折算为5 g?kg-1?d-1；假手术组和模型组给予等体积蒸馏水。采用免疫组化法检测各组给药后7、14、21 d缺血脑组织NF-κBp65和I-κB蛋白的表达情况。结果与模型组比较...

Objective To explore the effects of Buyang Huanwu decoction(BYHWD)on expressions of nuclear factor-κB p65(NF-κBp65)and its inhibitor( I-κB)in signal transduction of NF-κB in brain tissue of rats with focal cerebral ischemia injury. Methods 180 Sprague-Dawley(SD)rats were randomly divided into normal group,sham-operated group,model group,pynolidine dithiocarbamate(PDTC)group,minocycline(MC)group and BYHWD treatment group,each group 30 rats. The rats of PDTC group were given PDTC 100 mg?kg-1?d-1 by intra-peritoneal injection. In MC group,MC w...