期刊论文

Yu, T.-Z.

中文

生理学报

0371-0874

1998

50

6

623-628

10.3321/j.issn:0371-0874.1998.06.004

本校为其他机构

实验采用分离培养兔肺内小动脉平滑细胞(PASMC),观察低氧对PASMC的血管紧张素转化酶(ACE)活性和基因表达的影响.结果表明:(1)正常情况下培养的兔PASMC中有ACE基因表达并可检测到其活性,而培养基中未检测到ACE活性;(2)低氧6 h后,ACE基因表达变化不明显,但在随后的12、24、48h后表达明显增加,呈时间依赖性;(3)低氧24h后,PASMC细胞中及条件培养基中ACE活性均显著增加.上述结果提示:(1)基础状态下培养的兔PASMC存在ACE;(2)低氧可促进PASMC ACE活性和基因表达,这可能与低氧性肺动脉高压有关.

In order to observe the effect of hypoxia on pulmonary artery smooth muscle cells (PASMC), angiotensin-converting enzyme (ACE) activity was measured by colometry and the expression of ACE mRNA was detected by Northern blot, using rabbit isolated and cultured PASMC. The main results are as follows: (1) under normoxia both ACE activity and gene expression could be detected in PASMC; and (2) the expression level of ACE mRNA, after an initial latent period of 6 h hypoxia, began to show a marked increase with time in the next 12, 24 and 48 h. Exposure to hypoxia for 24 h resulted in an increase in ...