期刊论文

Effect of berberine combined with bone marrow mesenchymal stem cells on energy metabolism of HUVECs damaged by high glucose

中文

中国药理学通报

1001-1978

2016

32

11

1585-1591

10.3969/j.issn.1001-1978.2016.11.020

国家自然科学基金资助项目（No81573956 81273753） 中西医结合防治心脑疾病的相关基础研究湖南省高校科技创新团队开放基金项目（No2015CXTD-4） 中西结合基础湖南省“十二五”重点学科开放基金资助项目（No ZXJCXK201514） 湖南中医药大学中医经典理论与经方应用研究科技创新团队,致谢：感谢团队成员为该项目付出的辛勤劳动,感谢湖南中医药大学医学基础教学实验中心提供实验场地,感谢中西医结合心脑疾病防治湖南省重点实验室和中医方证研究转化医学湖南省高校重点实验室提供指标检测仪器!

本校为第一机构

目的研究黄连的主要有效成分小檗碱联合骨髓间充质干细胞对于高糖作用下血管内皮细胞能量代谢的影响。方法① MTT法检测细胞增殖以及活性情况；②流式细胞术检测细胞周期；③彗尾法检测细胞 DNA 损伤情况；④HPLC测定细胞中ATP、ADP、AMP含量,能荷EC值；⑤ RT-PCR法测定CCR、COX mRNA表达；⑥ Western blot法检测COX、CCR的蛋白表达。结果①高糖干预损伤后的血管内皮细胞活性明显降低、增殖能力减弱,S+G2期的细胞数量明显减少,出现一定程度的DNA损伤,而小檗碱、骨髓间充质干细胞及其联合使用均能不同程度提高细胞活性和增殖能力,增加S+G2期的细胞数量,促进DNA的修复( P

Aim To investigate the effects of berberine combined with bone marrow mesenchymal stem cells ( BM-MSCs) on the energy metabolism of human umbilical vein endothelial cells ( HUVECs ) under the condition of high glucose. Methods (DThe state of cell reproduction and cell proliferating activity were determined by MTT assay. (2)The cell cycle was detected by flow cytometry ( FCM ). (3) DNA damage of cells was measured by comet tail assay. (4)The contents of ATP, ADP and AMP were determined by high performance liquid chromatography ( HPLC ) and the level of energy charge (EC) was calculated. (5)The ...