期刊论文

中文

针刺研究

1000-0607

2017

42

5

429-433

10.13702/j.1000-0607.2017.05.010

国家重点基础研究发展计划“973”中医专项（No.2014CB 543102）；

本校为第一机构

目的:观察不同经穴组合针刺对失眠大鼠下丘脑生物钟基因Clock和Bmal 1表达的影响,探索循经选穴对腧穴配伍效应的影响。方法:SD雄性大鼠随机分为空白组(n=12)、模型组(n=8)、百会+神门组(n=12)、百会+三阴交组(n=11)、百会+非经非穴组(n=10)。连续2 d腹腔注射对氯苯丙氨酸(PCPA)混悬液(500 mg/kg)建立失眠模型。百会+神门组、百会+三阴交组、百会+非经非穴组每日针刺1次,每次30 min,连续7 d。治疗后比较各组大鼠睡眠潜伏期及睡眠时间;实时荧光定量PCR法检测下丘脑腹外侧视前区(VLPO)与视交叉上核(SCN)的Clock及Bmal 1 mRNA的表达水平。结果:与空白组比较,模型组睡眠潜伏期延长、睡眠时间减少(P

OBJECTIVE: To observe the effect of manual acupuncture stimulation (MAS) of "Baihui"(GV 20)-"Shenmen"(HT 7)or GV 20-"Sanyinjiao"(SP 6) on sleep and expression of circadian rhythm genes (Clock and Bmal 1) in the hypothalamus in insomnia rats, so as to select a better acupoint group for insomnia. METHODS: A total of 53 SD rats were randomly divided into normal control (n=12),insomnia model (n=8), GV 20-HT 7(n=12), GV 20-SP 6(n=11),and GV 20-non-acupoint (n=10) groups. The insomnia model was established by intraperitoneal injection of p-chlorophenylalanine (PCPA,500 mg/kg,100 mg/mL) once daily fo...