期刊论文

Effect and Mechanism of Curcumin Acetylsalicylate on Angiotensin Ⅱ Induced Proliferation in Vascular Smooth Muscle Cells

中文

湖南中医药大学学报

1674-070X

2017

37

11

1222-1225

10.3969/j.issn.1674-070X.2017.11.013

81673722:国家自然科学基金 14JJ1024:湖南省***基金 2015JJ2117:江苏省自然科学基金 16A156:湖南省教育厅重点项目

本校为第一机构

目的 探讨乙酰水杨酸姜黄素酯(curcumin acetylsalicylate,CA)对血管紧张素II(Angiotensin II,AngII)诱导的血管平滑肌细胞(vascular smooth muscle cells,VSMC)增殖的抑制作用及其相关机制.方法 建立AngII诱导的VSMC增殖模型.采用MTT法、流式细胞术观察CA干预对细胞活力和周期的影响.Western blot检测其对第10号染色体同源缺失性磷酸酶张力蛋白(phosphatase and tensin homolog deleted on chromosome ten,PTEN)、磷酸化AKT(p-AKT)、AKT蛋白表达的影响.结果 1、3、10μmol/L CA可以显著抑制AngII诱导的细胞活力,其中3μmol/L为最佳浓度(P

Objective To investigate the effect and mechanism of curcumin acetylsalicylate (CA) on Angiotensin II (AngII) induced proliferation in vascular smooth muscle cells (VSMC). Methods VSMC proliferation model was induced by AngI-I. Flow cytometry was used to observe the effect of CA on cell proliferation and cycle. The expressions of PTEN, p-AKT and AKT was detected by Western blot. Results 1, 3, 10 μmol/L CA led to a significant inhibition of Ang II-induced proliferation of VSMCs and 3 μmol/L CA was proved to be an optimal inhibitory concentrati...