期刊论文

The influence of Wuji Pill Solution on morphology and apoptosis of SGC-7901 cells infected with Helicobacter pylori in vitro

中文

佛山科学技术学院学报(自然科学版)

1008-0171

2015

4

67-70

10.13797/j.cnki.jfosu.1008-0171.2015.0082

湛江市科技攻关资助项目（2009C3101016）； 广东医学院青年基金资助项目（XQ0713）； 广东医学院面上基金资助项目（XK1445）； 广东省自然科学基金资助项目（S2012040007298）；

本校为其他机构

目的在体外研究戊己丸水煎液对幽门螺杆菌诱导的SGC-7901 细胞形态及凋亡的作用.方法以SGC-7901 细胞作为幽门螺杆菌株感染的体外细胞模型, 用不同浓度的戊己丸水煎液干预, 倒置显微镜观察SGC-7901细胞的形态;流式细胞仪分析胃上皮细胞SGC-7901的凋亡百分率.结果戊己丸水煎液加入幽门螺杆菌感染的细胞后,受损细胞出现修复;不同浓度戊己丸水煎液均可以减少幽门螺杆菌诱导的SGC-7901细胞的凋亡,与阴性对照组比较差异有显著性（p〈0.05）.结论戊己丸水煎液能降低幽门螺杆菌感染的SGC-7901细胞的凋亡率;对幽门螺杆菌感染的SGC-7901细胞的形态有保护作用.

Objective To investigate the effecte of Wuji Pill Solution on morphology and apoptosis of SGC- 7901 cells infected with Helicobacter pylori(H·pylori) in vitro. Methods H·pylori were introduced to the cells.Microscope was applied to observe the morphology and flow cytometry was used to analyze the extent of apoptosis of cells infected with H·pylori. Results The morphology of cells infected with H·pylori was repaired with Wuji Pill Solution. The apoptosis of cells were lower than that of contrast group( p