期刊论文

Determination of Gastrodin in Shenan Granules by HPLC

中文

江西中医药大学学报

2095-7785

2014

3

60-62

本校为第一机构

目的：建立以高效液相色谱法测定神安颗粒中天麻素含量的方法。方法：色谱柱为Kromasil C18柱，流动相为乙腈-0.05％磷酸溶液（0．5∶99．5），检测波长为220nm ，流速为1．0mL /min ，柱温为室温，进样量为10μL。结果：天麻素进样量在0.0518～0．5180μg范围内与峰面积积分值线性关系良好（r＝0．9993），平均回收率为98．05％（RSD＝2．92％）。结论：本方法简便、快捷、专属性强、重现性好，可用于神安颗粒苷类成分的质量控制。

Objective:To establish HPLC method for determination of Gastrodin in Shenan granules .Methods:HPLC was performed on Kromasil C18 column,the mobile phase consisted of acetonitrile -0.05% phosphoric acid solution (0.5∶99.5),the detection wave-length is 220 nm, flow rate 1.0mL /min, column temperature room temperature , sample size 10μL.Results:Gastrodin is linear with the peak area in the range of0.0518~0.5180μg (r=0.9993), the average recovery was 98.05%(RSD=2.92%).Conclusion:The established method is simple , shortcut, well specificity, repr...