Quantitative determination of 15 bioactive triterpenoid saponins in different parts of Acanthopanax henryi by HPLC with charged aerosol detection and confirmation by LC-ESI-TOF-MS

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成果类型：

期刊论文

作者：

Zhang, Xiao-dan;Li, Zhi;Liu, Guo-zhu;Wang, Xiang;Kwon, Ok-kyoung;...

通讯作者：

Liu, Xiang-qian

作者机构：

[Zhang, Xiao-dan] Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China.

[Wang, Xiang; Li, Zhi; Liu, Xiang-qian] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.

[Liu, Guo-zhu] Guangdong Drug Res Inst, Dongyangguang Grp, Dongguan, Peoples R China.

[Kwon, Ok-kyoung; Lee, Hyeong-kyu] Korea Res Inst Biosci & Biotechnol, Chungbuk, South Korea.

[Whang, Wan-kyunn] Chung Ang Univ, Pharmaceut Resources Bot Lab, Seoul, South Korea.

通讯机构：

[Liu, Xiang-qian] H

Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.

语种：

英文

关键词：

Acanthopanax henryi;Charged aerosol detection;Quality control;Triterpenoid saponins

期刊：

Journal of Separation Science

ISSN：

1615-9306

年：

2016

卷：

期：

页码：

2252-2262

DOI：

10.1002/jssc.201501029

基金类别：

Hunan Provincial Natural Science FoundationNatural Science Foundation of Hunan Province [11JJ2042]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LQ14H280006]; Science of Chinese Materia Medica of Twelfth Five-Year Key Discipline Projects of Hunan province; Changsha City Science and Technology Bureau Key Projects [K120701021]; Science of Pharmaceutical Analysis of Twelfth Five-Year Key Discipline Projects of Hunan University of Chinese Medicine

机构署名：

本校为通讯机构

院系归属：

药学院

摘要：

Triterpenoid saponins are difficult to analyze using high-performance liquid chromatography coupled to UV/vis spectrophotometry due to their lack of chromophores. This study describes the first analytical method for the determination of 15 triterpenoid saponins from the leaves, stems, root bark, and fruits of Acanthopanax henryi, using a high-performance liquid chromatography with charged aerosol detection coupled with electrospray ionization mass spectrometry method. The separation was carried out on a Kinetex XB-C18 column with an acetonitrile/water gradient as the mobile phase, followed by ...

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Quantitative determination of 15 bioactive triterpenoid saponins in different parts of Acanthopanax henryi by HPLC with charged aerosol detection and confirmation by LC-ESI-TOF-MS

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