期刊论文

An Experimental Study on the Oriented Differentiation Inducing Effect of Shenghuang Extract Solution on the Adult Rat Mesenchym Stem Cells into Neuron-Like Cells

中文

湖南中医药大学学报

1674-070X

2003

23

6

7-9

10.3969/j.issn.1674-070X.2003.06.003

湖南省自然科学基金资助项目 （ 0 2JJY40 46）；

本校为第一机构

目的探讨生黄醇提取液对成年大鼠骨髓间质干细胞(MSCs)的体外增殖和特异性诱导、分化为神经细胞的能力.方法采用生黄醇提取液、2-硫基乙醇(2-ME)、二甲基亚枫+成纤维细胞生长因子(DMSO+FGF)、1640培养液+15%FeS(对照)4组体外定向诱导MSCs向神经元细胞分化,用倒置显微镜和免疫组化技术检测神经元细胞所表达的特异性标志.结果3种不同诱导液均能定向诱导MSCs分化为神经元细胞,其中,生黄液组的NSE(神经元特异性烯醇化酶)和GFAP(胶质纤维酸性蛋白)的阳性细胞数明显高于2-ME和DMSO+FGF组(P＜0.05),1640培养液+15%FeS组没有诱导作用.结论MSCs能在体外扩增、传代、能被定向诱导分化为神经元细胞.

Objective To explore the potential ability of Shenghuang Extract Solution(SES) to induce oriented differentiation effect on the adult rat mesenchym stem cells(MSC S)into neuron-like cells(NLC S) and the ability of MSC S to proliferate in vitro. Method MSC S were orientedly induced to differentiate into NLC S in vitro, cultured in the media added with SES, 2-ME and DMSO plus FGF respectively and NLC S were identified by immunohistochemical techniques via determing the expression of specific markers on them.Results All the three kinds of inducing substance could induce oriented differentiation o...