GaoFei1,TanYuan1,MaLe2,HuangAo1,ZhangShuai1,LuoHong1 1DepartmentofDermatology,TheFirstHospitalofChangsha,Changsha410005,China;2Departmentof Dermatology,TheFirstHospitalofHunanUniversityofChineseMedicine,Changsha410005,China Correspondingauthor:LuoHong,Email:luohongyes@163.com 【 Abstract 】 Objective ToestablishaninvivodiffusionmodelofTreponemapallidum （ Tp ） in New Zealandrabbits.Methods AstandardstrainofTp（ Nichols strain ）wasrecoveredinthetestesofNew Zealand rabbits, and isolated and passaged continuously. The suspensions of the second⁃passage Tp were collected and inoculated onto the dorsal skin of New Zealand rabbits. After 21⁃day infection, the New Zealandrabbitswereanesthetizedandsacrificed,bloodsampleswerecollected,andskintissuesatthe infection site as well as liver, spleen, testes and lymph nodes were aseptically resected. Real⁃time fluorescence⁃based quantitative PCR was performed to detect the spread of Tp in different tissues and organs.Results Onday21afterinfectionwithTp,skinlesionssuchasindurationsandulcerswereseenat allinoculatedsitesofNewZealandrabbits.Pathologicalexaminationshowedalotofinflammatorycellsin the infected lesions, mainly including plasma cells, macrophages and lymphocytes. Real⁃time fluorescence⁃ basedquantitativePCRrevealedalargenumberofTpintissuesandorgans,suchasliver,spleenand testes.Conclusion AfterinoculationwithTpinthedorsalskinofNewZealandrabbits,Tpcouldspreadto theliver,spleen,testesandothertissuesandorgansthroughbloodandlymphnodes,andtheinvivo diffusionmodelofTpstrainsinNewZealandrabbitswassuccessfullyconstructed. 【 Key words 】 Treponemapallidum;Diseasemodels,animal;Rabbits;Pathologicprocesses Fund programs: Natural Science Foundation of Hunan Province of China（ 2019 JJ50535 ） ; Changsha ScienceandTechnologyPlanningProject （ kq 1901004 ） DOI: 10.35541/cjd.20201043
