期刊论文

中文

食品工业科技

1002-0306

2024

45

24

107-117

10.13386/j.issn1002-0306.2024010375

2023JJ30445:湖南省自然科学基金面上科学基金项目 22B0378:湖南省教育厅优秀青年项目 22JBZ053:湖南省中医药大学双一流建设学科揭榜挂帅项目 B2024012:湖南省中医药管理局科研项目

本校为第一机构

目的：从分子水平上揭示罗汉果甜苷单体与人类味觉受体之间的相互作用机制。方法：利用I-TASSER Server建立甜味受体（hT1R2、hT1R3）和苦味受体（hT2R4、hT2R14）的同源模型，依据ERRAT值、拉氏图、C-score等指标评估模型质量，选择最高质量的模型，通过AutoDock Vina模拟软件与Mogroside III、Mogroside IV、Mogroside V和Siamenoside I四种已知甜度罗汉果甜苷进行分子对接；构建罗汉果甜苷甜/苦味预测模型并对未知甜度罗汉果甜苷的甜/苦味进行预测；在此基础上，运用电子舌智能分析不同罗汉果甜苷单体的甜/苦味特征值，探究不同结构罗汉果甜苷单体与甜/苦味关键属性间的关系。结果：分析受体...

Objective:To reveal the interaction mechanism between mogroside monomers and human taste receptors at molecular level.Methods:The homologous models of sweet taste receptors(hT1R2,hT1R3)and bitter taste receptors(hT2R4,hT2R14)were established by I-TASSER Server.Model quality was evaluated according to ERRAT value,ramachandran plot,C-score and the model with the highest quality was selected.Molecular docking with Mogroside Ⅲ,Mogroside Ⅳ,Mogroside Ⅴ and Siamenoside Ⅰ were performed by AutoDock Vina simulation software.The sweet/bitter taste pr...