期刊论文

中文

中国中医药信息杂志

1005-5304

2014

21

2

23-26,31

10.3969/j.issn.1005-5304.2014.02.007

基金项目：国家自然科学基金（30973856）：湖南省中医药管理局科研重点项目（2010007）

本校为第一机构

目的探讨益气解毒方对中晚期鼻咽癌患者外周血CD4~+CD25~+调节性T细胞和Th17细胞的影响。方法用流式细胞仪检测18例经益气解毒方加常规治疗的中晚期鼻咽癌患者(观察组)及l5例常规治疗的中晚期鼻咽癌患者(对照组)外周血CD4~+CD25~+调节性T细胞和Th17细胞比例,RT-PCR法检测Foxp3 mRNA和ROR-γt mRNA转录水平,ELISA法检测血清白细胞介素-6(IL-6)和转化生长因子-β(TGF-β)水平。结果观察组患者外周血中CD4~+CD25~+调节性T细胞占CD4~+T细胞的比例和Foxp3 mRNA的相对表达量均低于对照组(P

Objective To investigate the effects of Qi-Boosting Toxin-Resolving Formula （QBTRF） on CD4＋CD25＋ regulatory T cells and Th17 cells of patients with middle to late staged nasopharyngeal carcinoma （NPC）. Methods Flow cytometry was performed to detect the ratio of CD4＋CD25＋ regulatory T ceUs and Th17 cells in the peripheral blood mononuclear ceUs （PMBC） among 18 patients with middle to late stage of NPC treated by QBTRF added to conventional therapy （treatment group）, Foxp3 mRNA and ROR-yt mRNA in PMBC was determined by RT-PCR techniqu...