期刊论文

Mechanism of activation of NLRP3 inflammasome byα-synuclein performed fibrils

中文

中国药物警戒

1672-8629

2023

20

04

409-415

10.19803/j.1672-8629.20220710

国家自然科学基金资助项目（82003973）；

本校为其他机构

目的探索纤维状α-突触核蛋白(α-synuclein)聚集体激活NLRP3炎症小体诱导神经炎症的机制。方法构建纤维状α-synuclein聚集体,采用纤维状α-synuclein聚集体刺激BV-2小胶质细胞,检测白介素(...展开更多 目的探索纤维状α-突触核蛋白(α-synuclein)聚集体激活NLRP3炎症小体诱导神经炎症的机制。方法构建纤维状α-synuclein聚集体,采用纤维状α-synuclein聚集体刺激BV-2小胶质细胞,检测白介素(IL)-1β、IL-18、IL-6和肿瘤坏死因子α(TNF-α)等相关炎症因子和NLRP3、caspase-1、ASC等蛋白的表达和mRNA水平评价NLRP3炎症小体激活;采用乳酸脱氢酶释放(LDH)实验检测细胞焦亡的发生。机制研究部分,检测...

Objective To investigate the mechanism by which fibrillarα-synuclein aggregates induce neuroinflammation by activating NLRP3 inflammasomes.Methodsα-synuclein performed fibrils(PFF)was established and added to BV-2 cells.Levels of such inflammatory factors as IL-1β,IL-18...MORE Objective To investigate the mechanism by which fibrillarα-synuclein aggregates induce neuroinflammation by activating NLRP3 inflammasomes.Methodsα-synuclein performed fibrils(PFF)was established and added to BV-2 cells.Levels of such inflammatory factors as IL-1β,I...