期刊论文

Effects of Senna on the intestinal microbiota and enzyme activity in mice with spleen-deficiency

中文

中国微生态学杂志

1005-376X

2018

30

2

155-157

10.13381/j.cnki.cjm.201802007

湖南省大学生研究性学习和创新性实验计划项目（湘教通[2016]283号）；

本校为第一机构

目的探讨灌胃番泻叶脾虚腹泻造模方法对小鼠肠道微生物及酶活性的影响。方法采用灌胃番泻叶水煎液进行小鼠脾虚造模。12只小鼠随机分为模型组和正常组,模型组灌胃番泻叶水煎液,正常组灌胃等量无菌水,期间观察小鼠的一般情况变化。灌胃7d后称重、无菌采集肠道内容物,分析肠道菌群及酶活性。结果与正常组相比,模型组小鼠肠道内容物的淀粉酶活性升高极显著（t=-53.0671,P=0.0000）,纤维素酶活性和木聚糖酶活性下降显著（t=23.1230,P=0.0000;t=12.3900,P=0.0410）,而蛋白酶活性变化不明显（t=1.3120,P=0.0521）;模型组小鼠细菌总数显著减少（t=73.1601,P=0.0000）,而大肠埃希菌、乳杆菌、双歧杆菌和...

Objective To explore the effects of Senna on gut microbes and enzyme activity in mice with spleen-deficiency.Methods Water decoction of Senna by gavage was adopted to make mouse models of spleen deficiency.Twelve experimental mice were randomly divided into the model group or control group,with 6 mice in each group.The model group was gavaged with water decoction of senna for 7 days,while the control group was given sterile water by gavage.The general changes of mice during the gavage were observed.After seven days,the mice were weighted and th...