期刊论文

Activity of sucrase in intestine of mice with dysbacteriosis diarrhea induced by antibiotics

中文

中国微生态学杂志

1005-376X

2018

30

9

1016-1019

10.13381/j.cnki.cjm.201809006

国家自然科学基础青年基金（81703985） 长沙市科技计划项目（kq1706057）

本校为第一机构

目的探究抗生素所致菌群失调腹泻小鼠肠道蔗糖酶的活性,为抗生素的临床应用提供参考。方法采用混合抗生素制备菌群失调腹泻小鼠模型,用DNS法测定造模期间小鼠肠道内容物及各肠段黏膜蔗糖酶的活性变化。结果造模4d后,模型组小鼠肠道内容物中蔗糖酶活性显著低于正常组。随着造模时间的延长,其蔗糖酶活性继续降低,远低于正常组。造模第2天起,模型组小鼠各肠段黏膜蔗糖酶活性均低于正常组,且随着造模时间延长,小鼠各肠段黏膜蔗糖酶活性降低程度有所不同,但与正常组相比差异均有统计学意义。结论抗生素可显著降低肠道内容物和肠黏膜蔗糖酶活性,抑制小鼠对营养物质的消化吸收功能。

Objective To explore the activity of sucrase in the intestine of mice with dysbacteriosis diarrhea induced by antibiotics and provide reference for rational clinical application of antibiotics.Methods Mouse models with dysbacteriosis diarrhea were established by administration of gentamicin sulfate and cefradine. Dynamic change of sucrase activity was monitored by using DNS method.Results The activity of sucrase in the intestinal contents of model mice decreased significantly compared with the control group after giving antibiotics for 4 days,which continued to decrease along with the days of ...