期刊论文

Effect of Tongxieyaofang prescription on intestinal microbial activity in mice with Ganqichenpi diarrhea

中文

中国微生态学杂志

1005-376X

2020

32

01

17-20

10.13381/j.cnki.cjm.202001004

81874460:国家自然科学基金 81703985:国家自然科学基金 :湖南中医药大学化学工程与技术一流学科

本校为第一机构

目的探讨痛泻要方对肝气乘脾泄泻小鼠肠道微生物活度的影响,为痛泻要方通过调节肠道微生物治疗肝气乘脾泄泻提供实验基础和理论依据。方法将25只SPF级雄性昆明小鼠随机分为正常组10只和模型组15只,造模成功后,随机选取正常组和模型组动物各5只,在无菌环境下提取肠道内容物和肠黏膜,剩余模型动物随机分为自然恢复组和治疗组各5只,治疗成功后处死各组动物提取肠道内容物和肠黏膜。用荧光素二乙酸法测定各样品中微生物活度。结果肝气乘脾泄泻造模小鼠肠道内容物微生物活度高于正常组,与正常组相比差异有统计学意义(P=0.01),而模型组小鼠肠黏膜微生物活度降低,与正常组相比差异有统计学意义(P=0.01)...

Objective To explore the effect of Tongxieyaofang prescription on intestinal microbial activity of mice with Ganqichenpi diarrhea,and provide experimental and theoretical basis for the treatment of the diarrhea with the formula by regulating intestinal microorganisms.Methods Twenty-five SPF male Kunming mice were randomly divided into the control group(n=10)or model group(n=15).After modeling,5mice each from the control group and model group were randomly selected to collect the intestinal contents and intestinal mucosa.The remaining model animals were randomly divided into natural recovery gr...