期刊论文

Effects of Tongxie Yaofang prescription on activity of digestive enzymes in intestinal mucosa and contents in Gan-qi-cheng-pi diarrhea mice

中文

中国微生态学杂志

1005-376X

2020

32

7

745-749

10.13381/j.cnki.cjm.202007001

国家自然科学基金(81703985)。

本校为第一机构

目的探讨痛泻要方对"肝气乘脾"泄泻小鼠肠道酶活性的影响。方法采用"番泻叶-离心管束缚夹尾法"进行"肝气乘脾"泄泻造模,造模成功后以痛泻要方治疗,造模和治疗后分别分析小鼠肠道酶活性。结果造模后,模型组小鼠肠道内容物的淀粉酶活性显著降低(t=4.007,P=0.015),纤维素酶、蛋白酶、蔗糖酶活性下降不显著。模型组小鼠肠黏膜蛋白酶、淀粉酶、蔗糖酶活性显著下降(t_蛋=5.652,P=0.005;Z_淀=-1.964,P=0.050;t_蔗=4.737,P=0.009)。痛泻要方治疗后,中药干预组小鼠肠道内容物蛋白酶、纤维素酶、乳糖酶和蔗糖酶活性变化不显著;自然恢复组的淀粉酶活性显著高于正常组(t=-7.497,P=0.002)。中药干预组小鼠肠道...

Objective To explore the effects of Tongxie Yaofang prescription on the activity of intestinal enzymes in mice with Gan-qi-cheng-pi (liver subjugating spleen) diarrhea caused by senna leaf plus restraint tail method. Methods Mouse models of Gan-qi-cheng-pi diarrhea were constructed by using lavage senna water decoction plus tail-clamping method.The mice were then treated with Tongxie Yaofang prescription.The activities of intestinal enzymes were analyzed after modeling and treatment respectively. Results After modeling, the activity of amylase in intestinal contents significantly decreased (t=...