期刊论文

中文

中国微生态学杂志

1005-376X

2023

35

11

1241-1247+1256

10.13381/j.cnki.cjm.202311001

2022JJ40332:湖南省自然科学基金项目 4901-020000200207:湖南中医药大学中医学国内一流建设学科项目

本校为第一机构

目的 通过枳实导滞汤剂对食积便秘小鼠肠道内容物和黏膜菌群的影响差异探讨肠道菌群在枳实导滞汤剂干预食积便秘中的作用.方法 采用网络药理学预测枳实导滞汤剂缓解便秘的药物成分和潜在功能;通过高脂高蛋白饮食结合腹腔注射盐酸洛哌丁胺制备食积便秘小鼠模型,造模成功后,治疗组小鼠给予枳实导滞汤剂0.4g/mL,正常组和模型组给予等剂量蒸馏水,每天2次,连续7d.治疗结束后,分别收集各组小鼠肠道内容物和黏膜,通过高通量测序技术分析肠道菌群.结果 网络药理学分析表明,从枳实导滞汤剂中共获得128个潜在活性成分,并获得1 379个便秘相关的靶点,药物和便秘检测到共同靶点74个.食积便秘小鼠肠道内容物与...

Objective To explore the role of intestinal flora in the intervention of Zhishi Daozhi decoction on dyspepsia constipation(DC)by analyzing the difference in the response of intestinal contents vs mucosal microbiota in mice with DC.Methods Network pharmacology was used to predict the drug composition and potential function of Zhishi Daozhi de-coction in relieving constipation.The models of DC were established by combining high-fat and high-protein diet with in-traperitoneal injection of loperamide hydrochloride.After the models were successfully established,the treatment group was given Zhishi ...