期刊论文

中文

中国微生态学杂志

1005-376X

2024

36

04

373-378+389

10.13381/j.cnki.cjm.202404001

2022JJ30440:湖南省教育厅重点科学项目 4901-020000200207:湖南中医药大学中医学国内一流建设学科 :中医学湖南省世界一流培育学科

本校为第一机构

目的 通过16SrRNA基因测序、网络药理学及分子对接技术探究杞菊地黄丸(QJDHP)对高脂诱导肥胖小鼠肠道黏膜菌群的影响.方法 15只SPF级雄性C57BL/6小鼠被分为正常组(C组,n=5)、模型组(HF组,n=5)及杞菊地黄丸组(QJDHP组,n=5).造模期间HF组与QJDHP组予高脂饲料喂养4个月,治疗期间QJDHP组灌胃QJDHP水煎液1个月,其余两组灌胃等量无菌水.结束后采集肠道黏膜,采用16S rRNA基因测序分析肠黏膜菌群,gutMGene数据库预测差异菌群代谢产物,网络药理学分析QJDHP干预肥胖的关键靶点,分子对接技术分析关键靶点与差异菌群代谢产物的结合能.结果 NMDS与ANOSIM结果显示三组间具有显著差异(stress=0.057 7;R=0.411,P...

Objective To observe the effect of Qi-Ji-Dihuang Pills(QJDHP)intervention on intestinal mucosal microbi-ota in high-fat diet-induced obese mice using 16S rRNA gene sequencing,network pharmacology,and molecular docking techniques.Methods A total of 15 SPF male C57BL/6 mice were divided into three groups:normal(C group,n=5),mod-el(HF group,n=5)and QJDHP group(n=5).The HF and QJDHP groups underwent four months of high-fat diet,with the latter receiving QJDHP via gavage for one month.Sterile water was administered to the remaining groups.After the inter-vention,intestinal mucosal samples were coll...