期刊论文

中文

时珍国医国药

1008-0805

2010

21

8

1865-1867

10.3969/j.issn.1008-0805.2010.08.008

基金项目：湖南省自然科学基金（No.00JJY2033）

本校为其他机构

目的探讨P38MAPK信号转导通路在β-榄香烯诱导人胃癌BAC823细胞凋亡过程中调控作用.方法人胃癌 BAC823细胞体外传代培养, 按空白对照组和药物处理组随机分组, 其中药物处理组依药物浓度的不同再分为0.01, 0.02, 0.04, 0.08 mg/ml, 0.10及0.16 mg/ml处理组, 作用时间为24h, 采用流式细胞光度分析术(FCM)检测细胞凋亡率及细胞周期时相分布, 同时进行细胞的形态学观察;采用免疫组化法检测P - P38MAPK的表达.结果B-榄香烯可阻滞 BAC823细胞于S期并诱导细胞凋亡, 但细胞凋亡率并不完全与药物浓度呈正相关.P38MAPK的活化水平随药物浓度的增加而增强.结论β-榄香烯可激活P38MAPK通路, 而且此通路也很可...

Objective To study the effect of β-elemene on signal transduction pathway P38MAPK during apoptosis of cancer cells.MethodsHuman gastric cancer BAC823 cells were cultured in vitro.The cells were divided into two groups including blank control group and drug groups.In terms of concentration,drug groups were divided into 0.01,0.02,0.04,0.08,0.10,and 0.16 mg/ml groups.The effective time was 24h.Apoptotic rate and fraction of the cell cycle phase were measured with flow cytometry（FCM）.At the same time,morphological and adhesive changes of the cel...