期刊论文

中文

时珍国医国药

1008-0805

2014

25

8

2016-2019

10.3969/j.issn.1008-0805.2014.08.090

国家自然科学基金（No.30472227）；

本校为其他机构

目的研究TNF-α和黄芩苷对人脐静脉血管内皮细胞(HUVEC)活力的影响及黄芩苷对体外TNF-α诱导培养的人脐静脉血管内皮细胞(HUVEC)凋亡的影响。以期从血管内皮细胞的角度探讨黄芩清热安胎的机理。方法选取不同浓度的TNF - α作用于HUVEC,四甲基偶氮唑盐(3-2,5-diphenyl - tetrazolium bromide,MTT)比色实验分析TNF - α对HUVEC细胞活力的影响。选取不同浓度的黄芩苷作用于HUVEC,培养24 h后,MTT比色实验分析黄芩苷对HUVEC细胞活力的影响。选取TNF - α作用浓度10ng/ml造模及原液1000倍稀释液的黄芩苷溶液作用于HUVEC,MTT比色实验和流式细胞术分析黄芩苷对HUVEC凋亡的影响。结果TNF-α作用后的HUVEC...

Objective To study the effects of TNF -α and baicalin on the activity of HUVEC. To research the effects of baicalin on HUVEC apoptosis induced by TNF - α. Methods HUVEC were cultured with different concentrations of TNF - α and baicalin. After culturing for 24h, the activity of HUVEC was examined by MTT assay . Flow cytometric analysis were used to determine the apoptotic cells. Results The activcty of the hepatocyte （A） in the groups of 10 ng ·ml^-1 and 50ng· ml^-1 of TNF-α were significantly lower than black control group （ P 〈0.05 ...