期刊论文

中文

时珍国医国药

1008-0805

2015

26

5

1064-1067

10.3969/j.issn.1008-0805.2015.05.015

国家重点基础研究发展计划(“973”计划)(No.2012CB723503) 湖南省科学技术厅科技计划一般项目(No.2013SK3107)

本校为第一机构

目的探讨脑健胶囊对局灶性脑缺血大鼠趋化因子单核细胞趋化蛋白- 1(MCP - 1)和巨噬细胞炎性蛋白- 1α (MIP - 1α)的影响。方法将100只大鼠随机分为假手术组、模型组、补阳还五汤组和脑健胶囊组,每组25只,采用大脑中动脉栓塞法建立永久性局灶性脑缺血模型,于术后1d、3d、7d、14d、28d处死,采用ELISA法检测血清中MCP - 1、MIP - 1α 含量,免疫组化法检测脑组织中MCP - 1、MIP - 1α 的阳性表达。结果模型组大鼠自术后1d始血清中MCP - 1、MIP - 1α 含量即较假手术组明显升高(P < 0.05或P < 0.01),脑健胶囊组大鼠血清中上述指标在各时间点均较模型组降低(P < 0.05或P < 0.01),且其1d的MCP - 1和14...

Objective To investigate the effect of Naojian capsules on the expression of chemokine monocyte chemoattractant protein-1( MCP- 1) and macrophage inflammatory protein- 1α( MIP- 1α) in rats after focal cerebral ischemia. Methods The rats were randomly divided into sham operation group,model group,BYHWD group and Naojian capsules group,each group contains25 rats. Using the method of middle cerebral artery occlusion( MCAO) to establish a permanent focal cerebral ischemia model.After surgery 1d,3d,7d,14 d,28d,the rats received different process a...