期刊论文

Effects of Different Processing Methods on the Content of Guanosine and Adenosine in Pinellia

中文

时珍国医国药

1008-0805

2017

28

9

2127-2129

10.3969/j.issn.1008-0805.2017.09.026

国家中医药管理局中药炮制学重点学科（国中医药人教发【2012】32号） 湖南省“十二五”重点学科中药学专业（湘教发[2011]76号） 湖南中医药大学校级研究生创新课题（No.2015CX20）

本校为第一机构

目的测定半夏不同炮制品(法半夏、姜半夏和清半夏)中鸟苷和腺苷的含量。方法采用高效液相色谱法; Ultimate XB - C_(18)色谱柱(4。 6mm × 250mm,0。 45μm);流动相:乙腈-水;流速: 1。 0ml /min;检测波长: 260nm;柱温: 30℃;采用梯度洗脱法测定半夏不同炮制品中鸟苷和腺苷的含量。结果半夏生品中鸟苷和腺苷含量均高于各炮制品。结论炮制会降低半夏中鸟苷和腺苷含量。

Objective To determine the different processed products of Pinellia (Rhizoma Pinellinae Praeparata,Pinellia Rhizoma Prepared by Ginger and Alumen and prepared Rhizoma Pinellinae without adjuvant) on the Content of Pinellia Guanosine and Adenosine. Methods An HPLC method was used on an Ultimate XB - C_(18) chromatographic column (4.6 mm × 250 mm,0.45 μm) with methanol - water as the mobile phase,the flow rate was 1.0 ml /min; the column temperature was at 30 ℃,and the detection wavelength was at 260 nm; Gradient elution method was used to det...