期刊论文

Effects of Wenyangzhenshuai Granule on miR-155 /P38MAPK regulates myocardial cell injury model

中文

时珍国医国药

1008-0805

2019

30

2

257-260

10.3969/j.issn.1008-0805.2019.02.001

81704061:国家自然科学基金 81173213:国家自然科学基金 201651:湖南省中医药局项目

本校为第一机构

目的 观察温阳振衰颗粒含药血清对H9C2心肌细胞损伤模型miR-155/P38MAPK表达的影响.方法 H9C2心肌细胞分为正常对照组,正常+含药血清组,转染组,转染+含药血清组,阿霉素(ADR)组,ADR+含药血清组,ADR+转染组,ADR+转染+含药血清组.ADR组的培养基中加入2.67 μmol/L的ADR,含药血清组培养基中加入10％的温阳振衰颗粒含药血清,共培养44h.检测实验末各组细胞miR-155和P38MAPK的表达水平.结果 与正常对照组相比,ADR组可显著下调miR-155的表达,同时上调P-P38MAPK的表达,差异具有统计学意义(P＜0.05).转染组和含药血清组可明显上调miR-155的表达,同时下调P-P38MAPK的表达,差异具有统计学意义(P＜0.05).结论...

Objective To elucidate the effects of Wenyangzhenshuai granule containing serum on miR-155/P38MAPK regulates H9C2 myocardial cell injury model. Methods H9C2 myocardial cells were divided into normal control group,normal+ drug serum group,transfection group,transfection+ drug serum group,ADR group,ADR+ drug serum group,ADR+ transfection group,ADR+ transfection+ drug serum drug. Adding 2.67 μmol/L ADR in H9C2 myocardial cells and 10% drug serum, coculture for 44 hours. miR-155 and P38MAPK expressions in these groups were detected by Western blot...