期刊论文

Protective effects and mechanism of Huangqi Siwu decoction nephrotic rats with iodine contrast agent nephropathy

中文

时珍国医国药

1008-0805

2019

30

7

1537-1540

10.3969/j.issn.1008-0805.2019.07.001

国家自然科学基金(81774208) 湖南省教育厅项目(17C1226) 湖南中医药大学校级课题(ZYYDX201749).

本校为第一机构

目的探讨黄芪四物汤对碘造影剂肾病(contrast-induced nephropathy,CIN)大鼠的保护作用及机制研究。方法24只2月龄雄性SD大鼠等量随机分成碘海醇模型组、黄芪四物汤组、水化组及正常对照组,通过注射碘海醇进行造模。通过灌胃于造模前48h,24h,造模后24h,36h灌注黄芪四物汤及等体积生理盐水,运用血生化法检测大鼠肾功能(血清肌酐、血清尿素氮),HE染色法观察肾脏结构变化,二步法免疫组化检测肾组织凋亡和抗凋亡因子Bax和Bcl-2蛋白的表达。结果各组大鼠的初始体重第1天不具有显著差异,第3天模型组、黄芪组、水化组体重与正常对照组相比均具有显著差异(P

Objective To investigate the protective effect and mechanism of Huangqi Siwu decoction on contra-induced nephropathy(CIN) rats.Methods 24 male SD rats of 2 months old were randomly divided into the iodil model group,the Huangqi siwu decoction group,the hydration group and the normal control group.The rat renal function (serum creatinine,serum urea nitrogen) was measured by blood biochemical method.The changes of renal structure were observed by HE staining.The expressions of renal tissue apoptosis and anti-apoptotic factor BAX and BCL-2 protein were detected by two-step immunohistochemistry.Re...