期刊论文

Optimization of extracting process and antibacterial effect in vitro of total glycosides in Qiwei Baizhu powder

中文

时珍国医国药

1008-0805

2020

31

06

1351-1354

10.3969/j.issn.1008-0805.2020.06.020

国家自然科学基金（81804076）； 湖南中医药大学校级大学生研究性学习和创新性实验计划课题（2018）；

本校为第一机构

目的比较优化七味白术散总苷的提取工艺及初步探究其体外抑菌活性。方法采用均匀设计法,以乙醇浓度、乙醇用量、提取时间、超声功率为考察因素,以总苷量及人参皂苷量加权得到的综合评分为评价指标,通过二次逐步回归分析筛选水提醇沉法及超声辅助提取法从七味白术散汤剂中提取总苷的最优提取工艺,并对七味白术散总苷在体外抑制大肠杆菌及酵母菌的活性进行初步研究。结果研究得到水提醇沉法的优化提取工艺为:用14倍量的68%乙醇提取15 h,该工艺的浸膏得率为29.28%,并得到总苷0.28 g,人参皂苷0.0871 g。超声辅助提取法的优化提取工艺为: 4倍量的56%乙醇在60%超声功率下提取40 min,浸膏得率为31.99 %...

Objective To optimize the optimal extraction conditions for total glycosides of Qiwei Baizhu powder. Methods Alcohol sedimentation and ultrasonic - assisted extraction were used. The extracting process was optimized by U6 * ( 64 ) uniform design. Ethanol concentration,ethanol consumption,extractive times and ultrasonic power were investigated as important factors. Extract rate,total glycosides and ginsenoside were considered as the evaluation indices. The antibacterial effect in vitro of total glycosides in Qiwei Baizhu powder was also considered. Results The optimization process were predicte...