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Acoustofluidic Salivary Exosome Isolation: A Liquid Biopsy Compatible Approach for Human Papillomavirus–Associated Oropharyngeal Cancer Detection

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成果类型:
期刊论文
作者:
Wang, Zeyu;Li, Feng;Rufo, Joseph;Chen, Chuyi;Yang, Shujie;...
通讯作者:
Huang, TJ;Wong, DTW
作者机构:
[Rufo, Joseph; Huang, TJ; Huang, Tony Jun; Wu, Mengxi; Yang, Shujie; Wang, Zeyu; Zhang, Jinxin; Chen, Chuyi] Duke Univ, Dept Mech Engn & Mat Sci, 144 Hudson Hall,Box 90300, Durham, NC 27708 USA.
[Kim, Yong; Tu, Michael; Li, Feng; Cheng, Jordan; Li, Liang; Wong, David T. W.] Univ Calif Los Angeles, David Geffen Sch Med, Ctr Oral Head & Neck Oncol Res, Los Angeles, CA 90095 USA.
[Abemayor, Elliot] Univ Calif Los Angeles, David Geffen Sch Med, Sch Dent, Los Angeles, CA 90095 USA.
[Abemayor, Elliot] Univ Calif Los Angeles, David Geffen Sch Med, Dept Otolaryngol Head & Neck Surg, Los Angeles, CA 90095 USA.
[Chia, David] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pathol, Los Angeles, CA 90095 USA.
通讯机构:
[Wong, DTW ] U
[Huang, TJ ] D
Duke Univ, Dept Mech Engn & Mat Sci, 144 Hudson Hall,Box 90300, Durham, NC 27708 USA.
Univ Calif Los Angeles, Ctr Oral Head & Neck Oncol Res, 10833 Le Conte Ave,73-017 CHS, Los Angeles, CA 90095 USA.
语种:
英文
关键词:
biological marker;RNA;virus DNA;microRNA;tumor marker;virus DNA;acoustics;Article;centrifugation;DNA sequence;exosome;human;Human papillomavirus type 16;human tissue;liquid biopsy;microfluidics;nonhuman;oropharynx cancer;Papillomaviridae;particle size;real time polymerase chain reaction;saliva;simulation;viscosity;chemistry;clinical trial;complication;exosome;genetics;Human papillomavirus type 16;isolation and purification;liquid biopsy;multicenter study;nucleotide sequence;oropharynx tumor;papillomavirus infection;pathology;polymerase chain reaction;procedures;prognosis;virology;Base Sequence;Biomarkers, Tumor;DNA, Viral;Exosomes;Human papillomavirus 16;Humans;Liquid Biopsy;Microfluidics;MicroRNAs;Oropharyngeal Neoplasms;Papillomavirus Infections;Polymerase Chain Reaction;Prognosis;Saliva;Viscosity
期刊:
JOURNAL OF MOLECULAR DIAGNOSTICS
ISSN:
1525-1578
年:
2020
卷:
22
期:
1
页码:
50-59
基金类别:
Supported by NIH grants UG3 TR002978 (D.T.W.W.), UH3 TR000923 (D.T.W.W.), U01 CA233370 (D.T.W.W.), UH2 CA206126 (D.T.W.W.), R01GM132603 (T.J.H.), R01 HD086325 (T.J.H.), and R21 CA239052 (D.T.W.W. and F.L.); United States Army Medical Research Acquisition Activity W81XWH-18-1-0242 (T.J.H); the Canadian Institute of Health (CIHR) Doctoral Foreign Student Award (J.C.); the Tobacco-Related Disease Research Program (TRDRP) Predoctoral Fellowship (J.C.); and the Queen Elizabeth Hospital Birmingham (QEHB) Charity UK and the Get-A-Head charity UK support the InHANSE (H.M.).
机构署名:
本校为其他机构
摘要:
Previous efforts to evaluate the detection of human papilloma viral (HPV) DNA in whole saliva as a diagnostic measure for HPV-associated oropharyngeal cancer (HPV-OPC) have not shown sufficient clinical performance. We hypothesize that salivary exosomes are packaged with HPV-associated biomarkers, and efficient enrichment of salivary exosomes through isolation can enhance diagnostic and prognostic performance for HPV-OPC. In this study, an acoustofluidic (the fusion of acoustics and microfluidics) platform was developed to perform size-based is...

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