期刊论文

Effect of Rhodioloside on the Protein Expressions of PI3-K,p-Akt and Caspase-3 in Rats with Cerebral Ischemia/Reperfusion Injury

中文

河南中医

1003-5028

2015

21

9

26-28

10.13862/j.cnki.cn43-1446/r.2015.09.010

(No.201329):湖南省中医药管理局重点资助项目 (No.2013126):湖南省中医药管理局一般资助项目

本校为第一机构

目的:观察红景天苷对大鼠脑缺血-再灌注损伤后PI3-K、p-Akt及Caspase-3表达的影响。方法:将60只SD雄性大鼠随机分为假手术组12只、脑缺血-再灌注组（模型组）及红景天苷组（10 mg/kg）各24只。采用线栓法制备大鼠右侧大脑中动脉缺血-再灌注模型,观察缺血2 h再灌注6 h、12 h、24 h、48 h 4个不同时间点,采用Western Blotting法检测PI3-K、p-Akt及Caspase-3的蛋白表达。结果:与假手术组比较,模型组及红景天苷组4个不同时间点PI3-K、p-Akt、Caspase-3蛋白表达量均显著升高,差异均有统计学意义（P

Objective： To observe the changes on the protein expressions of PI3-K,p-Akt and Caspase-3 in rats with cerebral ischemia/reperfusion injury. Methods： The 60 SD rats were randomly divided into three groups, the sham-operation group, the I/R group （the model group）, the rhodioloside treatment group （10mg/kg）. The model of middle cerebral artery occlusion was established by thread ligation method. Adult SD rats were observed cerebral ischemia for 2 h, and reperfusion at 6 h, 12 h, 24 h and 48 h respectively. The protein expressions of PI3-K,...