期刊论文

中文

湖南中医药大学学报

1674-070X

2017

37

8

813-818

10.3969/j.issn.1674-070X.2017.08.001

国家自然科学基金资助项目（81273665）； 湖南中医药大学中医诊断国家重点学科开放基金项目（2015ZYZD09）； 湖南省2011数字中医药协同创新中心；

本校为第一机构

目的运用i TRAQ定量蛋白质组学技术筛选慢性浅表性胃炎（chronic superficial gastritis,CSG）不同中医证候患者唾液中的潜在特异性生物标志物,探索一种客观化、规范化的中医辨证方法。方法采用i TRAQ定量蛋白质组学技术及后期生物信息学方法,筛选CSG脾虚证28例与CSG湿热证28例对比以及与正常对照组28例对比的唾液差异蛋白质分析。结果 CSG脾虚证对比CSG湿热证,Ratio≥1.5的共有128个蛋白,Ratio≤0.667的共有39个蛋白;CSG脾虚证对比正常对照组Ratio≥1.5的共有113个蛋白,Ratio≤0.667的共有72个蛋白。筛选出多个重要蛋白,如Keratin家族、ANX家族和apolipoprotein家族中的多种亚型以及γ-谷氨酸转...

Objective To screen the potential specific biomarker in saliva of chronic superficial gastritis (CSG) with different TCM syndromes by iTRAQ quantitative proteomics, and to explore the objective and standard TCM syn-dromes differentiation methods. Methods Saliva samples were taken from 28 cases of CSG patients with spleen deficiency syn-drome (group A), 28 cases of CSG patients with damp-heat syndrome (group B) and 28 cases of normal control volunteers (group C), in which differential expressed proteins were analyzed by iTRAQ quantitative proteo...