期刊论文

Ning, Y

英文

LUMINESCENCE

1522-7235

2020

35

3

347-354

10.1002/bio.3733

We would like to thank National Natural Science Foundation of China (81803964, 81874375), Hunan Key Laboratory Cultivation Base of Research and Development of Novel Pharmaceutical Preparations (2016TP1029), Natural Science Foundation of Hunan Province (2019JJ50693); Research Project Funded by Education Department of Hunan Province (No. 19C), Doctor start‐up Foundation of Hunan University of Chinese Medicine (No. 9982‐1001019), Key Subjects of Hunan University of Chinese Medicine《pathogenic biology》(NO.1) and Project Funded by Hunan Provincial Level Course《Immunology and pathogenic biology》(No. 48) for the financial support. We would like to thank National Natural Science Foundation of China (81803964, 81874375), Hunan Key Laboratory Cultivation Base of Research and Development of Novel Pharmaceutical Preparations (2016TP1029), Natural Science Foundation of Hunan Province (2019JJ50693); Research Project Funded by Education Department of Hunan Province (No. 19C), Doctor start-up Foundation of Hunan University of Chinese Medicine (No. 9982-1001019), Key Subjects of Hunan University of Chinese Medicine《pathogenic biology》(NO.1) and Project Funded by Hunan Provincial Level Course《Immunology and pathogenic biology》(No. 48) for the financial support.

本校为通讯机构

The development of a nanographite (NG)-based fluorescent biosensor for detecting microRNA (miRNA) is reported. Duplex-specific nuclease (DSN)-assisted signal amplification was key to its function. In the absence of a target, with the assistance of p-stacking interactions, the NG adsorbed the double carboxyfluorescein (FAM)-labelled probe (DFP) whose surface was perfectly complementary to miRNA, leading to quenching of FAM fluorescence. In the presence of a target, double-stranded DNA/RNA hybrids were repelled by the NG and fluorescence was rest...