期刊论文

Effects of Electroacupuncture at "Liangmen" (ST 21) and "Zusanli" (ST 36) on Dynamic Expression of Serum Metabolites in Acute Gastric Mucosal Injury Model Rats

中文

中医杂志

1001-1668

2019

60

7

602-607

10.13288/j.11-2166/r.2019.07.014

国家自然科学基金(81473751) 深圳市科技计划(JCYJ20160406140612883).

本校为其他机构

目的 采用核磁共振氢谱技术(1H NMR)探讨电针梁门-足三里治疗急性胃黏膜损伤的可能作用机制. 方法 54只SD大鼠随机分为正常组、模型组、电针组,各组又分为干预1、4、7天3个时间点,每个时间点6只.除正常组外其余各组采用75％乙醇灌胃法复制急性胃黏膜损伤大鼠模型.造模成功后电针组大鼠用鼠板捆绑固定,采用疏密波(4 Hz,50 Hz),电针“梁门”(负极)“足三里”(正极),每次30 min,每日1次.正常组和模型组大鼠用鼠板捆绑固定30 min,每日1次.分别于干预1、4、7天后光镜观察胃黏膜病理形态,检测大鼠血清1H NMR谱,并利用模式识别方法分析不同干预时间下代谢物谱的差异,用正交偏最小二乘判别分析(OPLS-DA)...

Objective To investigate the possible mechanism of electroacupuncture at Liangmen (ST 21) and Zusanli (ST 36) in the treatment of acute gastric mucosal injury by nuclear magnetic resonance spectroscopy (~~1H NMR). Methods A total of 54 SD rats were randomly divided into normal group, model group and electroacupuncture group. Each group was divided into three subgroup treated for 1, 4 and 7 days respectively, with 6 rats at each subgroup. Except for the normal group, the rat models of acute gastric mucosal injury were replicated by 75% ethanol. After successful modeling, the rats in the electro...