期刊论文

Urine Metabolomics Characteristics of Qi Deficiency Pattern Model Rats

中文

中医杂志

1001-1668

2021

62

06

516-522

10.13288/j.11-2166/r.2021.06.012

国家自然科学基金（81603512,81874429）； 湖南省自然科学基金（2019JJ40214）； 湖南省教育厅重点项目（19A380）； 湖南省中医药科研计划重点项目（202004）；

本校为第一机构

目的运用代谢组学方法探讨气虚证的潜在生物学基础。方法将20只SD大鼠随机分为正常对照组和气虚证模型组各10只,气虚证模型组大鼠采用限制饮食+游泳力竭法制备气虚证大鼠模型,正常对照组大鼠自由饮水、正常进食。运用超高效液相色谱-质谱联用（UHPLC-QE-MS）的代谢组学技术对各组大鼠尿液代谢物进行检测。通过SIMCA（V15.0.2）软件对差异代谢物进行主成分分析（PCA）和正交-偏最小二乘判别分析（OPLS-DA）,并绘制相对应的得分图。两组差异代谢产物进一步进行代谢路径拓扑分析,筛选富集性显著的代谢路径。结果与正常对照组比较,气虚证模型组的PCA与OPLS-DA得分图显示的代谢轮廓存在显著差异,气虚...

Objective To explore the potential biological basis of qi deficiency pattern through metabolomics. Methods Twenty SD rats were randomly divided into a normal control group and a qi-deficiency model group with 10 rats in each group. Restricted diet combined with exhausting swimming were used to develop a qi-deficiency model; rats in control group was free to drink water and take normal diet. Ultra-high performance liquid chromatography-mass spectrometry (UHPLC-QE-MS) was used to detect the urine metabolites of rats in each group. Principal component analysis (PCA) and orthogonal partial least s...