期刊论文

Li Lan-Fang

英文

生物化学与生物物理进展

1000-3282

2011

38

12

1153-1161

10.3724/SP.J.1206.2011.00334

supported by grants from The National Natural Scientific Foundation of China（30901577）； The Hengyang Technological Bureau of China（2009KJ14）； Ministry of Education of Returned Overseas Students to Start Research and Fund Projects（20091590）； Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province~~；

本校为其他机构

Previously, we found that G protein-coupled receptor APJ endogenous ligand apelin-13 stimulates vascular smooth muscle cells (VSMC) proliferation mediated in part by PKC-PI3K-ERK1/2-cyclinDl signaling cascades. In this study, Raf-1-14-3-3 signaling in rat VSMCs proliferation stimulated by apelin-13 was further investigated. Cell proliferation was measured with MTT assay. Expression of PI3K, phospho-PI3K, Raf-1, phospho-Raf-1, ERK1/2, phospho-ERKl/2, cyclinDl and cyclinE were detected by Western blotting. 14-3-3 protein combining with Raf-1 was ...

本室以前已经报道了G蛋白偶联受体APJ的内源性配体多肽,apelin-13,通过激活ERK1/2促进大鼠血管平滑肌细胞增殖.本文研究14-3-3信号蛋白是否参与apelin-13促进大鼠血管平滑肌细胞增殖ERK1/2信号途径,探讨apelin/APJ系统的细胞信号转导机制.组织贴块法培养大鼠胸主动脉VSMCs;Western blotting方法检测14-3-3、pRaf-1、Raf-1、pERK1/2、ERK1/2、cyclinD1、cyclinE的表达;MTT方法观察14-3-3抑制剂Difopein对VSMCs的增殖作用;免疫共沉淀方法检测14-3-3和Raf-1蛋白复合物的形成.Western blotting方法结果显示,apelin-13（0、0.5、1、2、4μmol/L）浓度依赖性刺激大鼠VSMCs 14-3-3表达、Raf-1和ERK1/2磷...