期刊论文

Guinea pig model of allergic diseases induced by Populus pollen

中文

中国组织化学与细胞化学杂志

1004-1850

2016

25

3

248-253

10.16705/j.cnki.1004-1850.2016.03.009

国家林业公益性行业科研专项（201304103） 湖南省“中药学”重点学科项目（湘教通[2011]76号）

本校为第一机构

目的 建立杨树花粉粗提物致敏激发的豚鼠过敏模型.方法 36只豚鼠随机分为正常组、卵清蛋白(OVA)阳性对照组和模型组,每隔5天经腹腔注射致敏1次,共3次,末次致敏后第5天雾化吸人激发.瑞氏染色观察支气管肺泡灌洗液(BALF)中炎症细胞变化,电脑视频计数200个细胞中嗜酸性粒细胞数目;常规病理切片HE染色观察鼻黏膜、与肺的炎症情况;免疫组化法检测肺组织中IL-4及IFN-γ阳性细胞平均光密度值;酶联免疫吸附试验检测血清中的总IgE、HIS、LTB4、IL-4及IFN-γ水平.结果 与正常组比较,OVA对照组、模型组均可诱导鼻黏膜及肺组织出现明显的变应性炎症;BALF涂片显示明显的炎症细胞(嗜酸性粒细胞、中性粒细胞)增...

Objective To develop a guinea pig model of allergic diseases induced by crude extractive of Populus pollen. Methods A total of 36 Dunkin Hartley guinea pigs were divided randomly into three groups: normal control group, Albumin Egg( OVA) group and Populus pollen model group with 12 samples in each group. Guinea pigs were repeatedly sensitized by intraperitoneal injections of OVA or crude Populus pollen extractive once every 5d for a total of three times. Five days after the last sensitization, guinea pigs were repeatedly placed in atomized antigen once daily for 5 days. The changes of inflamma...