期刊论文

Zinc finger transcription factor Sp8 is widely distributed in the olfactory bulb of adult rats and expressed in its interneurons

中文

中国组织化学与细胞化学杂志

1004-1850

2019

28

4

299-303

10.16705/j.cnki.1004-1850.2019.04.001

湖南省自然科学基金青年项目(2018JJ3517) 益阳市科技局应用基础项目(2017YR02) 湖南省教育厅优秀青年项目(16B269)。

本校为第一机构

目的探讨锌指结构转录调控因子Sp8在成年健康大鼠嗅球的分布及与嗅球中间神经元的关系。方法4月龄SD大鼠嗅球的冠状位切片,免疫荧光单标观察Sp8在嗅球的分布特点,免疫荧光双标观察Sp8与嗅球PV、TH、CB中间神经元的共表达。结果Sp8免疫阳性细胞在大鼠嗅球各层均有分布,其中主要分布于僧帽细胞层、内丛状层和颗粒细胞层,而在外丛状层最少;Sp8/PV和Sp8/CB双标细胞均主要分布在EPL层,Sp8/TH双标细胞主要分布在突触球层,共表达率均超过90%。结论Sp8广泛分布于嗅球且在其大多数中间神经元表达。

Objective To explore the distribution of zinc finger transcription factor Sp8 in the olfactory bulb of adult rats and its relationship with interneurons. Methods 4-month-old Sprague Dawley rats were sacrificed and their olfactory bulbs were sectioned into coronal slides. The distribution characteristics of Sp8 in the olfactory bulb were observed by single-labeled immunofluorescence staining and the co-expression of Sp8 with PV, TH and CB in interneurons was detected by double-labeled immunofluorescence. Results Sp8-positive cells were distributed in all layers of olfactory bulb, mainly distrib...