期刊论文

Screening Experiment of the Optimal TNBS and Ethanol Concentration in Rat Model of Crohn＇s Disease

中文

中国中医药现代远程教育

1672-2779

2018

16

18

72-75

10.3969/j.issn.1672-2779.2018.18.032

No.81603705:国家自然科学基金 No.81774438:国家自然科学基金 No.2017JJ3245:湖南省自然科学基金

本校为第一机构

目的 应用不同浓度TNBS与乙醇的混合液灌肠制备克罗恩病大鼠模型,对比结果进行评价研究,寻求制备克罗恩病大鼠最佳的TNBS与乙醇浓度,为克罗恩病的基础研究提供可靠的动物模型.方法 SPF级大鼠60只,采用分层随机法分为5组;分别予以体积比为1∶1的双蒸水和无水乙醇混合液、2∶1的TNBS和50％乙醇混合液、1∶1的TNBS和50％乙醇混合液、2∶1的TNBS和无水乙醇混合液、1∶1的TNBS和无水乙醇混合液按0.6 mL/只(100～150 mg/kg)灌肠,比较造模后大鼠不同的一般行为学、粪便及含水量的测定、体重、组织病理学等指标的差异.结果 与对照组比较,模型组大鼠出现精神状态差、粪便含水量高、结肠肉眼、镜下观均有不...

Objective To prepare a Crohn＇s d isease rat model by enema with different concentrations of TNBS and different concentrations of ethanol, and to evaluate the results of the comparison. To find out the optimal concentration of TNBS and ethanol for Crohn＇s disease rat, the basic research of grace provides a reliable animal model. Methods Sixty SPF rats were divided into 5 groups by stratified randomization method. The ratio of 1：1 mixture of distilled water and absolute ethanol, 2：1 TNBS and 50%ethanol were respectively used. 1：1 mixture of ...