期刊论文

Study on the Effect of Acupuncture on the Expression of VEGF in Brain Tissue of Rats with Cerebral Ischemia-reperfusion Injury Based on Time Point

中文

中国中医药现代远程教育

1672-2779

2020

18

20

116-119

10.3969/j.issn.1672-2779.2020.20.046

地方高校国家级大学生创新创业训练计划项目【No.201710541006】 湖南省中医药管理局一般项目【No.201961】。

本校为第一机构

目的通过观察针刺对大鼠脑缺血再灌注损伤后不同时间点VEGF表达的影响,探讨针刺对脑保护作用的可能机制。方法将雄性SD大鼠80只随机分为假手术组、模型组、针穴点对照组、针穴点组,再随机分成干预1、3 d组,10只/组,采用改良线栓法制备大脑中动脉缺血模型（MCAO）。治疗1 d与3 d后行神经功能缺损评分及TTC检测脑梗死面积比,蛋白印迹法检测大鼠缺血侧海马VEGF的表达。结果干预1 d后,相较于假手术组,造模组脑梗死面积比值升高明显（P

Objective To observe the effect of acupuncture on VEGF expression at different time points after cerebral ischemia-reperfusion injury in rats,and to explore the possible mechanism of acupuncture on brain protection.Methods 80 male SD rats were randomly divided into sham operation group,model group,acupoint control group and acupoint group.Each group was randomly divided into groups(1 d and 3 d);10/group.Middle cerebral artery ischemia model(MCAO)was established by modified thread occlusion method.After 1 d and 3 d of treatment,the ratio of infarct area was measured by neurological deficit scor...