The multicomponent qualitative and quantitative analysis of Stroma and Caterpillar body of Cordyceps sinensis were carried out by Heuristic Evolving Latent Projection (HELP) method. The procedure for resolving the two-way chromatogram/spectrum data obtained from the hyphenated instruments with HELP was developed in detail. It showed that HELP method could be used to examine the purity of chromatographic peaks, resolve overlapping peaks and finally obtain the pure chromatographic profiles and spectra for all the chemical components in the studied system. The separation ability of chromatography...