期刊论文

Effect of momordicin on caspase 3 in Balb/c mice myocarditis induced with CVB3

中文

中国医师杂志

1008-1372

2007

9

4

436-438

10.3760/cma.j.issn.1008-1372.2007.04.002

国家自然科学基金资助项目（30070934）；

本校为第一机构

目的 观察苦瓜素对病毒性心肌炎心肌组织caspase 3活性、基因转录及相应蛋白质表达的影响,探索苦瓜素治疗病毒性心肌炎的机制.方法 实验小鼠随机分为苦瓜素治疗组、生理盐水对照组及正常对照组和正常小鼠苦瓜素处理组.苦瓜素剂量为25 mg·kg-1·d-1,1次/d,疗程7 d.第15天后取小鼠心肌进行caspase 3活性测定、HE染色心肌病理检查、心肌细胞凋亡检查、RT-PCR检测、caspase 3,Mrna转录水平,免疫组化与免疫印迹测定caspase 3蛋白表达.结果 生理盐水对照组小鼠心肌组织caspase 3活性明显增高、Mrna转录水平与相应蛋白质表达水平亦显著增加;苦瓜素治疗组caspase 3活性明显抑制,caspase 3 Mrna转录水平...

Objective To observe the effect of momordicin on caspase 3 activation, mRNA transcription, and protein expression in the myocardium of Balb/c mice myocarditis induced with CVB3 , and to explore its therapeutical mechanism in myocarditis. Methods Fourweeks-old Balb/c mice induced with CVB 3 were randomly divided into four groups as follows： momordicin treatment group, vehicle control group, normal control group and momordicin group. Momordicin （25 mg · kg^-· d ^-1 ） were intraperitoneally administered daily from day 0 to 6. Caspase 3 activa...