期刊论文

The intervening effect of Qi-B0osting Toxin-Resolving Formula on migration potentiality of nasopha- ryngeal carcinoma cells and its association with the expression activities of cellular skeletal protein system

中文

中国医师杂志

1008-1372

2013

15

9

1153-1157

10.3760/cma.j.issn.1008-1372.2013.09.001

国家自然科学基金项目（81072904,30973856）； 湖南省科技厅项目（2009FJ3202,2012FJ6124）； 湖南省科技厅国际合作项目（06WK3016）； 湖南省教育厅项目（12C1257）； 湖南省海外名师计划（湘教通[2012]488号）；

本校为其他机构

目的 探讨益气解毒方对CNE2Z鼻咽癌细胞迁徙运动潜能的影响及其与细胞骨架蛋白系统表达活性的关系.方法 以正常大鼠血浆为空白对照,以益气解毒方大鼠血浆为干预因素,采用划痕法评价含药血浆对靶细胞CNE2Z鼻咽癌细胞迁徙运动潜能的影响,以扫描电镜观察干预前后靶细胞伪足形态特征,免疫组化SABC法检测β-tubulin、F-actin、p-Ezrin、Rac1表达活性,RT-PCR法检测p-Ezrin mRNA转录活性,评价靶细胞迁徙运动潜能变化与细胞骨架蛋白系统表达活性的关系及其对益气解毒方干预作用的反应性.结果 益气解毒方对CNE2Z鼻咽细胞的迁徙运动潜能显示抑制效应,在IC50浓度时,迁徙距离为(25.80 ±8.07) μm,抑制率为4...

Objective To investigate the intervening effect of Qi-Boosting Toxin-Resolving Formula (QBTRF) on the migration potentiality of nasopharyngeal carcinoma (NPC) cells and its association with the expressive activities of cellular skeletal protein system based on an experimental study in vitro.Methods In this study,QBTRF medicine-containing plasma of rats was taken as the intervening factor to treat the targeted NPC cells of CEN2Z for evaluating the correlation of changes in migrating potentiality of CEN2Z cells with expression activities of cellu...