期刊论文

中文

中国医师杂志

1008-1372

2019

21

03

364-367,372

10.3760/cma.j.issn.1008-1372.2019.03.012

湖南省教育厅基金（16C1208）~~；

本校为第一机构

目的研究胰岛素对糖尿病大鼠心肌缺血再灌注损伤磷脂酰肌醇3-激酶/蛋白激酶B（PI3K/Akt）信号通路的影响。方法采用高糖高脂饮食+腹腔注射链脲佐菌素（40 mg/kg）制备糖尿病大鼠模型,随机分为糖尿病假手术组（A组）、糖尿病缺血再灌注组（B组）、糖尿病缺血再灌注胰岛素处理组（C组）及糖尿病缺血再灌注胰岛素+Wortmaninn（PI3K抑制剂）处理组（D组）,每组10只。糖尿病大鼠心肌缺血再灌注模型:于左胸第三、四肋间暴露心脏,距左心耳下缘2 mm处用5-0无菌缝合线结扎左冠状动脉前降支（LAD）及心大静脉30 min,然后恢复灌注120 min。结扎前20min,D组经股静脉予Wortmannin（15μg/kg）,其余3组予等量的...

Objective To study the effect of insulin on phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway in diabetic rats with myocardial ischemia reperfusion injury.Methods Diabetic rats were induced by high-sugar and high-fat diet plus intraperitoneal injection of streptozotocin (40 mg/kg).They were randomly divided into diabetic sham group (group A),diabetes ischemic reperfusion group (group B),diabetes ischemic reperfusion insulin treatment group (group C) and diabetic ischemia reperfusion + insulin Wortmaninn (PI3K inhibitor...