期刊论文

Effects of GS-Rg1 on secretion of exosomes and expression of angiogenesis related miRNAs in mesenchymal stem cells

中文

中国医师杂志

1008-1372

2022

24

9

1312-1315,1320

10.3760/cma.j.cn431274-20220317-00192

国家自然科学基金（81904217）；

本校为其他机构

目的:探讨人参皂苷Rg1(GS-Rg1)调控间充质干细胞分泌外泌体(MSC-Exo)及对血管生成相关微小RNAs(miRNAs)表达的影响。方法:将人脐带血间充质干细胞(hUCBMSCs)分为实验组和对照组，实验组加入终浓度为40 mg/L的GS-Rg1，对照组加入等体积的PBS，均培养24 h。采用透射电子显微镜观察MSC-Exo形态，蛋白免疫印迹法鉴定其特征性表面标志物，二辛丁酸蛋白定量法检测MSC-Exo浓度，反转录聚合酶链反应(RT-PCR)检测MSC-Exo中8种与血管新生相关miRNAs的表达。结果:培养24 h后，可见呈圆形膜囊泡结构的MSC-Exo。MSC-Exo特征性表面标志物CD9、CD63和TSG101的表达均呈阳性。干预24 h后，实验组与对照组MSC-Exo蛋...

Objective:To investigate the effects of ginsenoside Rg1 (GS-Rg1) on the secretion of exosomes (MSC-Exo) and expression of angiogenesis related microRNAs (miRNAs) in mesenchymal stem cells.Methods:Human umbilical cord blood mesenchymal stem cells (hUCBMSCs) were divided into experimental group and control group. The experimental group was treated with GS-RG1 at a final concentration of 40 mg/L, while the control group was treated with phosphate buffered saline (PBS) at the same volume. Both groups were cultured for 24 h. The morphology of MSC-Ex...