期刊论文

Isolation and identification of active ingredients in Rubus corchorifolia L.f. Leaves and their bioactivities

Cao, Yong

中文

现代食品科技

1673-9078

2015

31

12

56-62

10.13982/j.mfst.1673-9078.2015.12.009

广东省科技计划农业公关项目(2012A020602038) 广州市科技计划重点项目(11C32100704) 广东省中医药局科研课题(2010276)

本校为其他机构

本文以山莓叶乙醇提取物为研究对象,通过溶剂萃取、硅胶柱层析、结晶等方法进行分离纯化。采用核磁共振（NMR）,质谱（MS）、红外光谱法（IR）及与文献对比的方法对其分离的单体进行结构鉴定。利用刃天青96孔板微量稀释法、MTT法研究单体化合物的抑菌及抗肿瘤活性。研究结果表明:从山莓叶中分离纯化物经结构鉴定分别为:三萜化合物类2α、3β、23α-三羟基-12-烯-28-乌苏酸（1）和黄酮类化合物山奈酚-3-O-β-D-（6’’-对羟基桂皮酰基）-葡萄糖苷（2）;抑菌实验表明化合物1对供试的大肠杆菌、痢疾志贺氏菌、金黄色葡萄球菌、沙门氏菌最低抑菌浓度分别为:15.6μg/m L、15.6μg/m L、31.25μg/m L、...

The ethanol extract of Rubus corchorifolia leaves was used in this study, and its active compounds were isolated and purified using solvent extraction, silica gel column chromatography, and crystallization methods. The structures of the isolated monomeric compounds were identified using nuclear magnetic resonance (NMR), mass spectrometry (MS), infrared (IR) spectroscopy, and comparisons with literature data. The antibacterial and antitumor activities of the monomeric compounds were studied by resazurin microdilution (96-well plate) assay and MT...