期刊论文

Tang, F.-Q.

中文

中南大学学报(医学版)

1672-7347

2004

29

5

577-582

10.3321/j.issn:1672-7347.2004.05.021

39770933,30171174:国家自然科学基金 95B063:国家中医药局科研项目 2003034496:中国博士后科学基金 :湖南省高校跨世纪学科带头人培养对象基金

本校为其他机构

目的:探讨中药复方益气解毒颗粒抑制鼻咽癌细胞裸鼠移植瘤生长的分子机制.方法:将鼻咽癌细胞系HNE1接种至BABL/c裸小鼠,20只,每只0.5×107个,待移植瘤生长至一定大小(约1 cm×1 cm×1 cm),随机分为两组.中药组灌喂益气解毒颗粒药液,对照组灌喂生理盐水,30 d后处死裸鼠,解剖获取肿瘤组织,测量瘤体体积大小,称量瘤体重量.取100 mg瘤体组织,常规提取总蛋白质,用固相pH梯度双向凝胶电泳分离移植瘤组织的总蛋白质,银染显色,ImageMaster 2D Elite 4.01软件分析差异蛋白质点.随机选取表达差异点共19个,4个点为只在中药组表达,4个点仅在对照中表达,余下11个为表达相差5倍以上的点,进行质谱鉴定和生物信息...

Objective: To investigate the inhibitory mechanism of Yiqijiedu granule on the implanted-tumor growth of nasopharyngeal carcinoma in nude mice. Methods: Twenty nude mice were injected nasopharyngeal carcinoma cells (HNE1), with 5 ×10 6 cells for a nude mouse. Implanted-tir mors grew for 20 d, whose volume reached 1 cm ×1 cm ×1 cm. These nude mice were divided into 2 groups: Yiqijiedu group and control group. The Yiqijiedu group was given Yiqijiedu granule, and the control was given normal saline for 30 d, and then were killed. The volume and...