期刊论文

Tian, H.

中文

中南大学学报(医学版)

1672-7347

2017

42

4

380-388

10.11817/j.issn.1672-7347.2017.04.003

(81303051):国家自然科学基金 (201471):湖南省中医药科研计划项目 (ZYNK201501):中医内科学省部共建教育部重点实验室资助 (81303051):This work was supported by the National Natural Science Foundation (201471):Traditional Chinese Medicine Science Research Project of Hunan Province (ZYNK201501):Province and Ministry Co-construction Key Laboratory for Internal Medicine of Traditional Chinese Medicine of the Ministry of Education :China

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目的:观察针刺联合亚低温对脑缺血再灌注大鼠脑组织MAPK/ERK通路及凋亡相关因子的影响,探讨针刺联合亚低温治疗缺血性中风的机制。方法:参照Longa线拴法复制并改良大脑中动脉缺血模型,将90只SD大鼠随机分为空白组、假手术组、模型组、针刺组、亚低温组、针刺联合亚低温组,针刺及亚低温治疗72 h后,观察神经功能缺损评分,采用2, 3, 5-苯氯化四氮唑(2, 3, 5-triphenyl-2H-tetrazolium chloride, TTC)染色检测梗死面积百分比,免疫组织化学法检测Bcl-2及Bax表达水平,TUNEL法检测凋亡细胞,Western印迹检测大鼠缺血侧海马组织MEK-2, ERK1/2蛋白的磷酸化水平。结果:造模后,模型组大鼠神经功能缺损评分增...

OBJECTIVE: To observe effect of acupuncture combined with hypothermia therapy on MAPK/ERK pathway and apoptosis related factorsin rats suffered cerebral ischemia reperfusion and to explore underlying mechanisms. Methods: Middle cerebral artery ischemia model were established.Ninety SD rats were randomly assigned into a blank group, a control group, a model group, an acupuncture group, a mild hypothermia group, and an acupuncture with hypothermia group. After 72 h treatment, nerve function defect scores were observed, and infarction area percent was detected by 2, 3, 5-triphenyl-2H-tetrazolium ...