期刊论文

Characteristics of electrophysiological changes in the process of astrocytes pyroptosis after hyperoxia exposure

中文

中南大学学报(医学版)

1672-7347

2020

45

7

759-765

10.11817/j.issn.1672-7347.2020.200029

81671506:国家自然科学基金 14JJ4019:湖南省自然科学基金

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目的:观察高氧环境下星形胶质细胞焦亡过程中的电生理学变化特征及其与细胞形态变化的相关性。方法:星形胶质细胞暴露于氧浓度为90%的常压高氧环境中12～72 h,应用全细胞膜片钳技术分别于不同时间点检测各组细胞的电生理活动特性,并观察相应时间点的细胞形态学变化,然后收集同批次细胞,应用蛋白质印迹法检测其caspase-1,caspase-3,功能蛋白（gasdermin,GSDM）D和GSDME表达水平。结果:常压高氧暴露后12～72 h,全细胞膜片钳记录到内向电流较对照组明显降低（P

Objective:To observe the electrophysiological changes of astrocytes in the process of hyperoxia induced apoptosis and analyze the relationship between electrophysiological characteristics and morphological changes.Methods:Astrocytes were exposed to 90% hyperoxia for 12-72 h.The electrophysiological characteristics of astrocytes in each group were detected by patch clamp technique, and the morphological characteristics of astrocytes were observed at the same time.Then the same batch of astrocytes were collected, and the expression levels of caspase-1, caspase-3, gasdermin D(GSDMD)and gasdermin ...