期刊论文

MicroRNA-539 inhibiting the occurrence and development of hepatocellular carcinoma by targeting E2F transcription factor 3

中文

解剖学报

0529-1356

2018

49

4

469-474

10.16098/j.issn.0529-1356.2018.04.009

本校为其他机构

目的探讨肝癌组织中microRNA-539(miR-539)表达水平及其抑制肝癌细胞增殖的作用机制。方法收集90例肝癌患者术后肝癌组织及癌旁组织标本,用实时定量聚合酶链反应(Real-time PCR)检测组织中miR-539表达量。在PCL/PRF5、Huh7和QGY-7701肝癌细胞中转染miR-539模拟物(mimics) ,用Real-time PCR检测miR-539表达量。用MTT法检测miR-539对肝癌细胞增殖的影响;用流式细胞仪检测miR-539对肝癌细胞凋亡的影响;用免疫印迹法检测miR-539对肝癌细胞中E2F转录因子3(E2F3)蛋白表达的影响。结果肝癌组织中miR-539表达量显著低于癌旁组织(P < 0.05) ; miR-539表达量与肝癌患者性别、年龄及肝癌组织分化程度无关(P...

Objective To investigate the expression of microRNA-539 (miR-539) in hepatocellular carcinoma (HCC) and the possible mechanism of miR-539 inhibition cancer cells. Methods Ninety cases of liver cancer patients after liver cancer tissue and adjacent tissue specimens were collected,and the expression of miR-539 in clinical tissues was detected by Real-time PCR. MiR-539 mimics was transfected into QGY-7701,Huh7 and PCL/PRF5 hepatoma cells,and the expression of miR-539 was detected by Real-time PCR. The effect of miR-539 on the proliferation of HCC cells was detected by MTT assay. The effect of miR...