期刊论文

Yuan, Z.-K.

中文

Chinese Journal of Tissue Engineering Research

1673-8225

2010

14

14

2497-2502

10.3969/j.issn.1673-8225.2010.14.006

国家自然科学基金资助项目（30772696） 课题名称“养心通脉有效部位方对大鼠心肌缺血骨髓间充质干细胞移植的影响” 湖南研究生创新基金资助项目（CX2009B168） 课题名称“心血瘀阻证微环境变化及对骨髓间充质干细胞移植的影响”

本校为第一且通讯机构

背景: 骨髓间充质干细胞在培养过程中, 常在基础培养基中添加一定的血清, 最常见的是小牛血清和胎牛血清, 但这存在着一定的生物安全性隐患. 目的: 观察不同血清微环境对大鼠骨髓间充质干细胞体外培养的影响. 方法: 自成年大鼠骨髓中分离骨髓间充质干细胞, 采用全骨髓贴壁法培养. 分别以下列血清微环境培养: 自身血清组: 分离细胞后原代用含大鼠自身血清的培养基培养, 传代后换用含胎牛血清的培养基培养; 同种异体血清组: 分离细胞后原代用含同种异体血清的培养基培养, 以后用胎牛血清培养基培养; 胎牛血清组: 分离后用含胎牛血清培养基培养, 以后一直用含胎牛血清培养基培养; DMEM组: 分离细胞...

BACKGROUND: During culture of bone marrow mesenchymal stem cells (BMSCs), a certain serum is commonly added in the basic medium, such as calf serum and fetal bovine serum, but there are potential biological safety risks. OBJECTIVE: To study the effects of different serum microenvironments on in vitro culture of rat BMSCs. METHODS: BMSCs were harvested from adult rat bone marrow, and cultured in vitro by whole bone marrow adherence method. The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium wi...