期刊论文

Peng, Q.-H.

中文

国际眼科杂志

1672-5123

2018

18

9

1572-1577

10.3980/j.issn.1672-5123.2018.9.04

Foundation items: National Natural Science Foundation of China ( No. 81774372 ); Research Project of Hunan Provincial Administration of Traditional Chinese Medicine (No. 201622); Research and Innovation Project for Graduate Students in Hunan ( No. CX2016B377, CX2017B432 ); Hunan Provincial Key Laboratory Project for Preventing and Treating Ophthalmology and Otorhinolaryngology (No. 2017TP1018); Science and Technology Plan of Changsha City (No. kc1704005); The Central Finance Supporting Local University Construction Projects; Key Discipline Project of State Administration of Traditional Chinese Medicine of Ophthalmology of TCM; Key Discipline Project of Hunan Province of Otorhinolaryngology of TCM 1Key Discipline of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China;2Key Discipline of Chinese Medical Ophthalmology, The Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China; 3Xi蒺an Hospital of Traditional Chinese Medicine, Xi蒺an 710021, Shaanxi Province, China Correspondence to:Qing-Hua Peng. Key Discipline of Chinese Medical Ophthalmology, The Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China. pqh410007@ 126. com Received:2018-04-12摇 摇 Accepted:2018-08-08

本校为第一且通讯机构

目的:观察活血散结中药复方含药血浆对血小板源性生长因子(platelet derived growth factor,PDGF)干预下兔RPE细胞增殖的影响.方法:酶消化法获取RPE原代细胞,进行RPE细胞的原代培养和传代;制备活血散结中药复方含药血浆;选取第4代兔RPE细胞为实验细胞,PDGF低、中、高剂量(5、10、20μg/L)干预48h后,CCK-8法检测并选取适宜细胞实验干预浓度;建立PDGF干预下RPE细胞增殖模型.实验分组为空白对照组(DMEM)、 正常血浆组、PDGF(10μg/L)组、PDGF(10μg/L)+AG1296(10μmol/L)组、PDGF(10μg/L)+10％ 含药血浆组,PDGF(10μg/L)+20％含药血浆组,分别加入相应处理因素干预24h后,Transwell法测定兔RPE细胞迁...

AIM: To invesitigate the effect of invigorating blood and dissipating masses traditional Chinese medicine compound drug-containing plasma on the proliferation of rabbit retinal pigment epithelium (RPE) cells treated with platelet derived growth factor (PDGF). METHODS: Primary cells of RPE cells were obtained by enzymatic digestion, and the primitive culture and subculture of RPE cells were proceeded; prepared blood plasma-contained traditional Chinese medicine compound drug-containing plasma; the fourth genertation rabbit RPE cells were selecte...