期刊论文

Li, D.

中文

国际眼科杂志

1672-5123

2021

21

02

217-221

10.3980/j.issn.1672-5123.2021.2.05

National Nature Science Foundation of China (No.81674030); Scientific Research Project of Hunan Development and Reform Commission (No.xcqz [2016] No.65); National College Students' Innovation and Entrepreneurship Training Program (No.201910541023); Hunan College Students' Research Learning and Innovative Experiment Program (No.2019-1670).

本校为第一机构

目的:通过体外分离及培养泪腺上皮细胞,建立干眼细胞模型,分析相关炎症因子,为进一步探究干眼治疗的有效药物奠定基础.方法:体外分离培养兔泪腺上皮细胞,并采用细胞增殖实验和免疫荧光实验对原代细胞活性和纯度进行鉴定.根据脂多糖(LPS)和肿瘤坏死因子-α(TNF-α)的IC50值,采用两者的0.5倍IC50刺激兔泪腺上皮细胞,构建干眼细胞模型即LPS组和TNF-α组,并通过细胞增殖实验、ELISA和流式方法对比两种构建干眼细胞模型的相关生物学特征.结果:兔泪腺上皮原代细胞培养12h后细胞基本贴壁,48h可见细胞形态舒展,呈长三角形.兔泪腺上皮原代细胞活性为92％以上,标志角化蛋白(Pan-cytoeratin)阳性率>90％,符...

AIM: To further explore effective drugs for dry eye treatment by isolating and culturing lacrimal gland epithelial cells in vitro, establishing a dry eye cell model and analyzing relevant inflammatory factors. METHODS: Rabbit lacrimal gland epithelial cells were in vitro isolated and cultured, and the activity and purity of primary cells were identified by cell proliferation experiment and immunofluorescence experiment. In addition, 0.5 times IC50 of lipopolysaccharide LPS and TNF-α were used respectively to stimulate rabbit lacrimal gland epi...